Split Inteins: Nature’s Protein Ligases
نویسندگان
چکیده
منابع مشابه
Streamlined Expressed Protein Ligation Using Split Inteins
Chemically modified proteins are invaluable tools for studying the molecular details of biological processes, and they also hold great potential as new therapeutic agents. Several methods have been developed for the site-specific modification of proteins, one of the most widely used being expressed protein ligation (EPL) in which a recombinant α-thioester is ligated to an N-terminal Cys-contain...
متن کاملTraceless protein splicing utilizing evolved split inteins.
Split inteins are parasitic genetic elements frequently found inserted into reading frames of essential proteins. Their association and excision restore host protein function through a protein self-splicing reaction. They have gained an increasingly important role in the chemical modification of proteins to create cyclical, segmentally labeled, and fluorescently tagged proteins. Ideally, intein...
متن کاملProtein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins
Protein trans-splicing by split inteins has many uses in protein production and research. Splicing proteins with synthetic peptides, which employs atypical split inteins, is particularly useful for site-specific protein modifications and labeling, because the synthetic peptide can be made to contain a variety of unnatural amino acids and chemical modifications. For this purpose, atypical split ...
متن کاملStructure-based engineering and comparison of novel split inteins for protein ligation.
Protein splicing is an autocatalytic process involving self-excision of an internal protein domain, the intein, and concomitant ligation of the two flanking sequences, the exteins, with a peptide bond. Protein splicing can also take place in trans by naturally split inteins or artificially split inteins, ligating the exteins on two different polypeptide chains into one polypeptide chain. Protei...
متن کاملSplit-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
BACKGROUND Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within live cells or in vivo. However one of the major problems regarding the use of QDs for biological imaging is the difficulty of targeting QDs onto proteins. We have recently dev...
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ژورنال
عنوان ژورنال: Israel Journal of Chemistry
سال: 2011
ISSN: 0021-2148
DOI: 10.1002/ijch.201100094